Versatile Antagonistic Activities of Soil-Borne Bacillus spp. and Pseudomonas spp. against Phytophthora infestans and Other Potato Pathogens.
Identifieur interne : 000618 ( Main/Exploration ); précédent : 000617; suivant : 000619Versatile Antagonistic Activities of Soil-Borne Bacillus spp. and Pseudomonas spp. against Phytophthora infestans and Other Potato Pathogens.
Auteurs : Simon Caulier [Belgique] ; Annika Gillis [Belgique] ; Gil Colau [Belgique] ; Florent Licciardi [Belgique] ; Maxime Liépin [Belgique] ; Nicolas Desoignies [Belgique] ; Pauline Modrie [Belgique] ; Anne Legrève [Belgique] ; Jacques Mahillon [Belgique] ; Claude Bragard [Belgique]Source :
- Frontiers in microbiology [ 1664-302X ] ; 2018.
Abstract
The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of Bacillus-like and Pseudomonas-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens Alternaria solani, Fusarium solani (BCCM-MUCL 5492), Pectobacterium carotovorum (ATCC 15713), Phytophthora infestans (CRA-W10022) and Rhizoctonia solani (BCCM-MUCL 51929) were performed, allowing the selection of 52 Bacillus spp. and eight Pseudomonas spp. displaying growth inhibition of at least 50% under in vitro conditions, particularly against P. infestans. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of Bacillus pumilus strains toward P. infestans, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven Bacillus spp. and four Pseudomonas spp.) in order to evaluate their in vivo antagonistic effect against P. infestans. Based on these results, four strains (Bacillus amyloliquefaciens 17A-B3, Bacillus subtilis 30B-B6, Pseudomonas brenneri 43R-P1 and Pseudomonas protegens 44R-P8) were retained for further evaluation of their protection index against P. infestans in a pilot field trial. Interestingly, B. subtilis 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.
DOI: 10.3389/fmicb.2018.00143
PubMed: 29487574
PubMed Central: PMC5816801
Affiliations:
- Belgique
- Province du Brabant wallon, Région wallonne
- Louvain-la-Neuve
- Université catholique de Louvain
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Versatile Antagonistic Activities of Soil-Borne <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. against <i>Phytophthora infestans</i> and Other Potato Pathogens.</title><author><name sortKey="Caulier, Simon" sort="Caulier, Simon" uniqKey="Caulier S" first="Simon" last="Caulier">Simon Caulier</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant 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wallon</region></placeName></affiliation></author><author><name sortKey="Desoignies, Nicolas" sort="Desoignies, Nicolas" uniqKey="Desoignies N" first="Nicolas" last="Desoignies">Nicolas Desoignies</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Modrie, Pauline" sort="Modrie, Pauline" uniqKey="Modrie P" first="Pauline" last="Modrie">Pauline Modrie</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Legreve, Anne" sort="Legreve, Anne" uniqKey="Legreve A" first="Anne" last="Legrève">Anne Legrève</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, 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type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2018">2018</date><idno type="RBID">pubmed:29487574</idno><idno type="pmid">29487574</idno><idno type="doi">10.3389/fmicb.2018.00143</idno><idno type="pmc">PMC5816801</idno><idno type="wicri:Area/Main/Corpus">000818</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000818</idno><idno type="wicri:Area/Main/Curation">000818</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000818</idno><idno type="wicri:Area/Main/Exploration">000818</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Versatile Antagonistic Activities of Soil-Borne <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. against <i>Phytophthora infestans</i> and Other Potato Pathogens.</title><author><name sortKey="Caulier, Simon" sort="Caulier, Simon" uniqKey="Caulier S" first="Simon" last="Caulier">Simon Caulier</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Gillis, Annika" sort="Gillis, Annika" uniqKey="Gillis A" first="Annika" last="Gillis">Annika Gillis</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Colau, Gil" sort="Colau, Gil" uniqKey="Colau G" first="Gil" last="Colau">Gil Colau</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Licciardi, Florent" sort="Licciardi, Florent" uniqKey="Licciardi F" first="Florent" last="Licciardi">Florent Licciardi</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Liepin, Maxime" sort="Liepin, Maxime" uniqKey="Liepin M" first="Maxime" last="Liépin">Maxime Liépin</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Desoignies, Nicolas" sort="Desoignies, Nicolas" uniqKey="Desoignies N" first="Nicolas" last="Desoignies">Nicolas Desoignies</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Modrie, Pauline" sort="Modrie, Pauline" uniqKey="Modrie P" first="Pauline" last="Modrie">Pauline Modrie</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Legreve, Anne" sort="Legreve, Anne" uniqKey="Legreve A" first="Anne" last="Legrève">Anne Legrève</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Mahillon, Jacques" sort="Mahillon, Jacques" uniqKey="Mahillon J" first="Jacques" last="Mahillon">Jacques Mahillon</name><affiliation wicri:level="4"><nlm:affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author><author><name sortKey="Bragard, Claude" sort="Bragard, Claude" uniqKey="Bragard C" first="Claude" last="Bragard">Claude Bragard</name><affiliation wicri:level="4"><nlm:affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve</wicri:regionArea><orgName type="university">Université catholique de Louvain</orgName><placeName><settlement type="city">Louvain-la-Neuve</settlement><region type="region" nuts="1">Région wallonne</region><region type="province" nuts="1">Province du Brabant wallon</region></placeName></affiliation></author></analytic><series><title level="j">Frontiers in microbiology</title><idno type="ISSN">1664-302X</idno><imprint><date when="2018" type="published">2018</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of <i>Bacillus</i>-like and <i>Pseudomonas</i>-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens <i>Alternaria solani, Fusarium solani</i> (BCCM-MUCL 5492), <i>Pectobacterium carotovorum</i> (ATCC 15713), <i>Phytophthora infestans</i> (CRA-W10022) and <i>Rhizoctonia solani</i> (BCCM-MUCL 51929) were performed, allowing the selection of 52 <i>Bacillus</i> spp. and eight <i>Pseudomonas</i> spp. displaying growth inhibition of at least 50% under <i>in vitro</i> conditions, particularly against <i>P. infestans</i>. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of <i>Bacillus pumilus</i> strains toward <i>P. infestans</i>, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven <i>Bacillus</i> spp. and four <i>Pseudomonas</i> spp.) in order to evaluate their <i>in vivo</i> antagonistic effect against <i>P. infestans</i>. Based on these results, four strains (<i>Bacillus amyloliquefaciens</i> 17A-B3, <i>Bacillus subtilis</i> 30B-B6, <i>Pseudomonas brenneri</i> 43R-P1 and <i>Pseudomonas protegens</i> 44R-P8) were retained for further evaluation of their protection index against <i>P. infestans</i> in a pilot field trial. Interestingly, <i>B. subtilis</i> 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.</div></front></TEI><pubmed><MedlineCitation Status="PubMed-not-MEDLINE" Owner="NLM"><PMID Version="1">29487574</PMID><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Electronic-eCollection"><Journal><ISSN IssnType="Print">1664-302X</ISSN><JournalIssue CitedMedium="Print"><Volume>9</Volume><PubDate><Year>2018</Year></PubDate></JournalIssue><Title>Frontiers in microbiology</Title><ISOAbbreviation>Front Microbiol</ISOAbbreviation></Journal><ArticleTitle>Versatile Antagonistic Activities of Soil-Borne <i>Bacillus</i> spp. and <i>Pseudomonas</i> spp. against <i>Phytophthora infestans</i> and Other Potato Pathogens.</ArticleTitle><Pagination><MedlinePgn>143</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.3389/fmicb.2018.00143</ELocationID><Abstract><AbstractText>The world potato is facing major economic losses due to disease pressure and environmental concerns regarding pesticides use. This work aims at addressing these two issues by isolating indigenous bacteria that can be integrated into pest management strategies. More than 2,800 strains of <i>Bacillus</i>-like and <i>Pseudomonas</i>-like were isolated from several soils and substrates associated with potato agro-systems in Belgium. Screenings for antagonistic activities against the potato pathogens <i>Alternaria solani, Fusarium solani</i> (BCCM-MUCL 5492), <i>Pectobacterium carotovorum</i> (ATCC 15713), <i>Phytophthora infestans</i> (CRA-W10022) and <i>Rhizoctonia solani</i> (BCCM-MUCL 51929) were performed, allowing the selection of 52 <i>Bacillus</i> spp. and eight <i>Pseudomonas</i> spp. displaying growth inhibition of at least 50% under <i>in vitro</i> conditions, particularly against <i>P. infestans</i>. All 60 bacterial isolates were identified based on 16S rRNA gene sequencing and further characterized for the production of potential bio-active secondary metabolites. The antagonistic activities displayed by the selected strains indicated that versatile metabolites can be produced by the strains. For instance, the detection of genes involved bacilysin biosynthesis was correlated with the strong antagonism of <i>Bacillus pumilus</i> strains toward <i>P. infestans</i>, whereas the production of both bio-surfactants and siderophores might explain the high antagonistic activities against late blight. Greenhouse assays with potato plants were performed with the most effective strains (seven <i>Bacillus</i> spp. and four <i>Pseudomonas</i> spp.) in order to evaluate their <i>in vivo</i> antagonistic effect against <i>P. infestans</i>. Based on these results, four strains (<i>Bacillus amyloliquefaciens</i> 17A-B3, <i>Bacillus subtilis</i> 30B-B6, <i>Pseudomonas brenneri</i> 43R-P1 and <i>Pseudomonas protegens</i> 44R-P8) were retained for further evaluation of their protection index against <i>P. infestans</i> in a pilot field trial. Interestingly, <i>B. subtilis</i> 30B-B6 was shown to significantly decrease late blight severity throughout the crop season. Overall, this study showed that antagonistic indigenous soil bacteria can offer an alternative to the indiscriminate use of pesticide in potato agro-systems.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Caulier</LastName><ForeName>Simon</ForeName><Initials>S</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo><AffiliationInfo><Affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Gillis</LastName><ForeName>Annika</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Colau</LastName><ForeName>Gil</ForeName><Initials>G</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Licciardi</LastName><ForeName>Florent</ForeName><Initials>F</Initials><AffiliationInfo><Affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Liépin</LastName><ForeName>Maxime</ForeName><Initials>M</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Desoignies</LastName><ForeName>Nicolas</ForeName><Initials>N</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Modrie</LastName><ForeName>Pauline</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Legrève</LastName><ForeName>Anne</ForeName><Initials>A</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Mahillon</LastName><ForeName>Jacques</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Laboratory of Food and Environmental Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Bragard</LastName><ForeName>Claude</ForeName><Initials>C</Initials><AffiliationInfo><Affiliation>Phytopathology-Applied Microbiology, Earth and Life Institute, Université Catholique de Louvain, Louvain-la-Neuve, Belgium.</Affiliation></AffiliationInfo></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2018</Year><Month>02</Month><Day>13</Day></ArticleDate></Article><MedlineJournalInfo><Country>Switzerland</Country><MedlineTA>Front Microbiol</MedlineTA><NlmUniqueID>101548977</NlmUniqueID><ISSNLinking>1664-302X</ISSNLinking></MedlineJournalInfo><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Bacillus spp.</Keyword><Keyword MajorTopicYN="N">Phytophthora infestans</Keyword><Keyword MajorTopicYN="N">Pseudomonas spp.</Keyword><Keyword MajorTopicYN="N">bacilysin</Keyword><Keyword MajorTopicYN="N">bio-control</Keyword><Keyword MajorTopicYN="N">lipopeptides</Keyword><Keyword MajorTopicYN="N">potato diseases</Keyword><Keyword MajorTopicYN="N">siderophores</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2017</Year><Month>08</Month><Day>21</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2018</Year><Month>01</Month><Day>23</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2018</Year><Month>3</Month><Day>1</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate 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IdType="pubmed">22029651</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province du Brabant wallon</li><li>Région wallonne</li></region><settlement><li>Louvain-la-Neuve</li></settlement><orgName><li>Université catholique de Louvain</li></orgName></list><tree><country name="Belgique"><region name="Région wallonne"><name sortKey="Caulier, Simon" sort="Caulier, Simon" uniqKey="Caulier S" first="Simon" last="Caulier">Simon Caulier</name></region><name sortKey="Bragard, Claude" sort="Bragard, Claude" uniqKey="Bragard C" first="Claude" last="Bragard">Claude Bragard</name><name sortKey="Caulier, Simon" sort="Caulier, Simon" uniqKey="Caulier S" first="Simon" last="Caulier">Simon Caulier</name><name sortKey="Colau, Gil" sort="Colau, Gil" uniqKey="Colau G" first="Gil" last="Colau">Gil Colau</name><name sortKey="Desoignies, Nicolas" sort="Desoignies, Nicolas" uniqKey="Desoignies N" first="Nicolas" last="Desoignies">Nicolas Desoignies</name><name sortKey="Gillis, Annika" sort="Gillis, Annika" uniqKey="Gillis A" first="Annika" last="Gillis">Annika Gillis</name><name sortKey="Legreve, Anne" sort="Legreve, Anne" uniqKey="Legreve A" first="Anne" last="Legrève">Anne Legrève</name><name sortKey="Licciardi, Florent" sort="Licciardi, Florent" uniqKey="Licciardi F" first="Florent" last="Licciardi">Florent Licciardi</name><name sortKey="Liepin, Maxime" sort="Liepin, Maxime" uniqKey="Liepin M" first="Maxime" last="Liépin">Maxime Liépin</name><name sortKey="Mahillon, Jacques" sort="Mahillon, Jacques" uniqKey="Mahillon J" first="Jacques" last="Mahillon">Jacques Mahillon</name><name sortKey="Modrie, Pauline" sort="Modrie, Pauline" uniqKey="Modrie P" first="Pauline" last="Modrie">Pauline Modrie</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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